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Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic condition, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin but not for the Schwann cell marker S-100.
RPNF from ScienCell Research Laboratories are isolated from CD® IGS rat nerve. RPNF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RPNF are characterized by immunofluorescence with antibodies specific to vimentin and/or fibronectin. RPNF are negative for mycoplasma, bacteria, yeast, and fungi. RPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RPNF in vitro.
货号 | R1710 |
产地 | 北美 |
缩写 | RPNF |
规格 | 5 x 10^5 cells/vial |
用途 | 科研 |
储存 | 液氮 |
运输 | 干冰 |
大鼠细胞系统
大鼠细胞系统
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R2540 | 大鼠淋巴单核细胞 | 10 x 10^6 cells/vial | $324 | + | |
R2670 | 大鼠唾腺成纤维细胞 | 5 x 10^5 cells/vial | $452 | + | |
R3040 | 大鼠肺泡巨噬细胞 | 1 x 10^6 cells/vial | $361 | + | |
R3200 | 大鼠肺泡上皮细胞 | 1 x 10^6 cells/vial | $633 | + | |
R3300 | 大鼠肺成纤维细胞 | 5 x 10^5 cells/vial | $452 | + | |
R4100 | 大鼠肾小管上皮细胞 | 5 x 10^5 cells/vial | $452 | + | |
R4200 | 大鼠肾系膜细胞 | 5 x 10^5 cells/vial | $452 | + | |
R5300 | 大鼠肝星状细胞 | 5 x 10^5 cells/vial | $484 | + | |
R5300-a | 大鼠肝星状细胞-成年 | 5 x 10^5 cells/vial | $484 | + | |
R5340 | 大鼠肝巨噬细胞 | 1 x 10^6 cells/vial | $376 | + | |
R5540 | 大鼠脾细胞 | 10 x 10^6 cells/vial | $195 | + | |
R5550 | 大鼠脾脏巨噬细胞 | 1 x 10^6 cells/vial | $376 | + | |
R6110 | 大鼠主动脉平滑肌细胞 | 5 x 10^5 cells/vial | $452 | + | |
R6200 | 大鼠心肌细胞 | 1 x 10^6 cells/vial | $452 | + | |
R6300 | 大鼠心肌成纤维细胞 | 5 x 10^5 cells/vial | $452 | + | |
R6520 | 大鼠角膜细胞 | 5 x 10^5 cells/vial | $452 | + | |
R6550 | 大鼠晶状体上皮细胞 | 5 x 10^5 cells/vial | $452 | + | |
R7500 | 大鼠骨髓间充质干细胞 | 5 x 10^5 cells/vial | $452 | + | |
R7500-1 | 大鼠骨髓间充质干细胞 | 1 x 10^6 cells/vial | $721 | + | |
R7540 | 大鼠肺间充质干细胞 | 1 x 10^6 cells/vial | $149 | + |