The bladder is a hollow ball, with the wall of the bladder composed of the serosa, muscularis, submucosa, musculris mucosa, and lamina propria. The endothelial cells lining the microvasculature of the circulatory system in the bladder are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues. Endothelial cells in the bladder wall stimulated with thrombin or tryptase causes phospholipid metabolite production and may contribute to the inflammatory process in interstitial cystitis. HBdMEC provide a useful in vitro model to study the role of endothelial cells in physiological and inflammatory conditions. HBdMEC from ScienCell Research Laboratories are isolated from human bladder tissue. HBdMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HBdMEC are characterized by immunofluorescence with antibodies specific to VWF/Factor VIII and/or CD31 (PECAM1). HBdMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdMEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HBdMEC.