ScienCell's GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit (GQH-PSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
- Pancreatic stellate cell markers: ACTA2, GFAP, COL11A1, DES, NGFR, POSTN, ITGA7, CTGF
- Pancreatic stellate cell activation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, CDH2, RARA, PDGFs, TGFB1, MMP2
- Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s
- ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
- Cytokine production: IL1A, IL1B, IL6, PDGFs, TGFBs, TNF
- p53 signaling pathway: CCNB1, CDK1, CCNE1, RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3
- Pancreatic fibrosis and cancer development: ACVR1B, CCKBR, CDKN2A, CELSR3, EGFR, GNL3, PALLD, RBBP8, RIMS1, SMAD4, STK11
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Plate Layout*
(8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ACTA2 |
CDK1 |
COL2A1 |
DSP |
HIF1A |
LAMA2 |
MMP13 |
PALLD |
RBP1 |
SPARC |
THBS2 |
β-actin |
B |
ACTG2 |
CDK6 |
COL4A2 |
EGFR |
ICAM1 |
LAMA3 |
MMP2 |
PDGFA |
RBP2 |
SPP1 |
TIMP1 |
GAPDH |
C |
ACVR1B |
CDKN1A |
CRABP1 |
EI24 |
IL1A |
LAMB1 |
MYH11 |
PDGFB |
RBP4 |
STK11 |
TNC |
LDHA |
D |
CCKBR |
CDKN2A |
CRABP2 |
FOXF1 |
IL1B |
LAMB3 |
MYH9 |
PDGFC |
RBP5 |
SYP |
TNF |
NONO |
E |
CCNB1 |
CELSR3 |
CTGF |
GADD45G |
IL6 |
LAMC1 |
NES |
PDGFD |
RELN |
TGFB1 |
VCAN |
PPIH |
F |
CCNE1 |
CHEK1 |
CYP26A1 |
GFAP |
ITGA7 |
LHX2 |
NFKB1 |
POSTN |
RIMS1 |
TGFB2 |
VIM |
GDC |
G |
CDH1 |
CLEC3B |
CYP26B1 |
GNL3 |
KLF6 |
LOX |
NFKB2 |
RARA |
RRM2 |
TGFB3 |
VTN |
PPC |
H |
CDH2 |
COL11A1 |
DES |
HAS1 |
LAMA1 |
LRAT |
NGFR |
RBBP8 |
SMAD4 |
TGFBI |
ZMAT3 |
NTC |
* gene selection may be updated based on new research and development
货号 | GK092 |
产地 | 美国 |
缩写 | GQH-PSB |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |
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