Renal glomerular endothelial cells (GEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are involved in pathophysiological processes in the glomerulum. GEC constitutively produce bioactive molecules, which can be amplified by inflammatory and thrombotic molecules. Endothelial cell injury due to a severe glomerular lesion can inhibit angiogenesis and result in sclerosis at the injured site. GEC injury affects mesangial and epithelial cells and leads to the progression of renal disease. The biological properties of GEC remain largely unknown because of difficulties associated with the culturing, cloning and propagation of these cells. HRGEC from ScienCell Research Laboratories are isolated from human kidneys. HRGEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HRGEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HRGEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRGEC are guaranteed to expand for 5 population doublings under the conditions specified by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HRGEC in vitro.