ScienCell's GeneQuery™ Human ECM Degradation qPCR Array Kit (GQH-ECM) profiles 88 key genes involved in extracellular matrix (ECM) degradation. The ECM comprises molecules secreted by cells that provide structural and biochemical support. Its roles include supporting intercellular communication, growth factor storage, and regulating cell migration and motility. Degradation of the ECM thus influences growth factor release, chemotaxis, cell migration, and cellular adhesion. Enzymes classically involved in ECM degradation include serine proteases, threonine proteases, and matrix metalloproteinases. Brief examples of how included genes may be categorized are shown below:
- ECM components: ACAN, COL18A1, COL4A1, FGG, LAMA5, NTN4
- Proteases: ADAM10, ADAMTS1, BMP2, CAPN9, CTSG, MMP2, MMP9
- Protease inhibitors: A2M, CAST, SERPINH1, TIMP1, TIMP2
- ECM proteoglycans: BGN, DCN, LTBP2, LUM, PCOLCE
- Cell adhesion receptors and glycoproteins: CD44, CEACAM8, FN1, HSPG2, SPARC
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human ECM Degradation qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
A2M |
ADAMTS4 |
CAPN1 |
CEACAM8 |
COL4A1 |
CTSG |
FGB |
LAMA2 |
LOX |
MMP3 |
PLG |
β-actin |
|
B |
ACAN |
ADAMTS5 |
CAPN10 |
COL11A1 |
COL4A3 |
CTSS |
FGG |
LAMA3 |
LTBP2 |
MMP7 |
PSEN1 |
GAPDH |
|
C |
ADAM10 |
BGN |
CAPN2 |
COL14A1 |
COL4A5 |
CTSV |
FN1 |
LAMA5 |
LUM |
MMP8 |
SERPINH1 |
LDHA |
|
D |
ADAM12 |
BMP1 |
CAPN3 |
COL18A1 |
COL5A1 |
DCN |
HSPG2 |
LAMB1 |
MMP1 |
MMP9 |
SPARC |
NONO |
|
E |
ADAM15 |
BMP10 |
CAPN9 |
COL1A1 |
COL5A2 |
DMD |
HTRA1 |
LAMB2 |
MMP12 |
NCSTN |
SPP1 |
PPIH |
|
F |
ADAM17 |
BMP2 |
CASP3 |
COL1A2 |
COL6A1 |
DMP1 |
KLK12 |
LAMB3 |
MMP13 |
NID1 |
TIMP1 |
GDC |
|
G |
ADAM8 |
BMP4 |
CAST |
COL2A1 |
COL6A3 |
FBN1 |
KLK7 |
LAMC1 |
MMP14 |
NTN4 |
TIMP2 |
PPC |
|
H |
ADAMTS1 |
BMP7 |
CD44 |
COL3A1 |
COL7A1 |
FGA |
LAMA1 |
LAMC2 |
MMP2 |
PCOLCE |
VCAN |
NTC |
* gene selection may be updated based on new research and development
| 货号 | GK105 |
| 产地 | 美国 |
| 缩写 | GQH-ECM |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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