ScienCell’s One-Step TaqProbe RT-qPCR Master Mix, 4x (TPRTMM) is designed for sensitive RNA detection and quantification by probe-based reverse transcription quantitative PCR (RT-qPCR). It contains optimized components that enable cDNA synthesis and PCR amplification to be performed and quantified in a single tube. The ready-to-use 4x master mix contains UNG/dUTP (to eliminate PCR carryover contamination), engineered M-MLV reverse transcriptase, Taq polymerase, dNTPs, and a buffer system for robust reverse transcription and qPCR.