ScienCell’s First-Strand cDNA Synthesis Master Mix, 4x (FSDMM) is optimized to synthesize the first strand cDNA from total RNA or mRNA. ScienCell’s engineered reverse transcriptase has mutations which increase sensitivity and thermostability, improve specificity, and reduce RNase H activity. The ready-to-use 4x master mix contains dNTPs, buffer, ScienCell’s engineered reverse transcriptase, RNase inhibitor and both random hexamer and anchored oligo (dT) primers in only one tube. Simply mix template RNA and water with FSDMM to begin the reaction.