ScienCell’s SapphireNStart TaqProbe qPCR Master Mix (STQMM) is a probe-based qPCR master mix with a “hot-start” property. STQMM is ideal for use in real-time quantitative PCR. The 2X master mix contains dNTPs, Taq DNA polymerase, and an inert blue-color loading indicator (ScienCell, catalog #GQ300G) in a single tube. The “hot-start” property achieved through ScienCell’s unique chemically modified Taq DNA polymerase provides maximal inhibition of non-specific signal formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert Sapphire-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes.