The coronary arteries provide a substantial blood supply to meet the enormous energy demands of the heart for its constant contractile activity. Lining the vessel wall, coronary artery endothelial cells (CAEC) are continuously exposed to fluid shear stress that induces changes in cell morphology and the production of endothelium-derived substances regulating vasoconstriction and vessel growth. CAEC also modulate the expression of cellular adhesion molecules to control and fine-tune inflammatory responses and fibrinolysis. These physiological properties allow CAEC cultures to be widely used in the study of mechanisms for endothelium dysfunction, pathogenesis of coronary heart disease and atherosclerosis, and the development of novel disease treatments. HCAEC from ScienCell Research Laboratories are isolated from human coronary artery. HCAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCAEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HCAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCAEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HCAEC in vitro.