ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation. HIF1 activity is a major component of the human response to hypoxia. Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:
- Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2
- Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1
- Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF
- Metabolism: PKM, STC2, DHX35, DDX11, ERO1A
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABCB1 |
BHLHE40 |
COL18A1 |
DUSP6 |
HMOX1 |
LOXL2 |
NEK4 |
PKM |
PRC1 |
RRAS |
TSFM |
β-actin |
|
B |
ADORA2A |
BRCA1 |
COL1A1 |
ELK1 |
IGFBP3 |
MAP3K5 |
NOTCH4 |
PLCG2 |
PSME3 |
RRM2 |
TXNIP |
GAPDH |
|
C |
ADORA2B |
CCNE1 |
COL1A2 |
ERO1A |
INHBA |
MCM4 |
P4HA1 |
PLOD1 |
PTGS1 |
SKP2 |
UBE2S |
LDHA |
|
D |
AK3 |
CCNE2 |
COL5A1 |
EXOSC2 |
INHBE |
MEF2A |
P4HA2 |
PLOD2 |
PTPRB |
SMAD9 |
VEGFA |
NONO |
|
E |
ANGPTL4 |
CCNF |
CXCR4 |
FOS |
INSR |
MLLT10 |
PAM |
POLE2 |
PTPRR |
STC1 |
VEGFC |
PPIH |
|
F |
BARD1 |
CDC6 |
DDX11 |
GADD45B |
KDM3A |
MMP2 |
PDZD2 |
POLR3K |
RB1 |
STC2 |
VLDLR |
GDC |
|
G |
BCL2 |
CDK1 |
DHFR |
GINS2 |
LEPR |
MXI1 |
PELI2 |
PPARD |
RFC5 |
TFAM |
ZNF292 |
PPC |
|
H |
BCL6 |
CDKN1C |
DHX35 |
HES1 |
LOX |
NDRG1 |
PGF |
PPARG |
RNH1 |
TRIP13 |
ZWINT |
NTC |
*gene selection may be updated based on new research and development
| 货号 | GK036 |
| 产地 | 美国 |
| 缩写 | GQH-HFT |
| 规格 | One 96-well plate, one 20ul reaction per well |
| 用途 | 科研 |
| 储存 | 4°C |
| 运输 | 胶冰 |
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