The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Despite great variability in size and shape, all neurons share common morphologic features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The human brain contains about 1 x 10^11 neurons and each are able to contact at least 10,000 other neurons. The hippocampal neurons play a special role in learning and memory. Cultured hippocampal neurons are a useful model for studying neuronal phenomena such as differentiation, survival, process growth, and synaptogenesis. HN-h from ScienCell Research Laboratories are isolated from human hippocampus. HN-h are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HNh are characterized by immunofluorescence with antibodies specific to β-tubulin III. HN-h are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN-h are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HNh are not recommended for expanding or long-term cultures since the cells do not proliferate in culture. Recommended Medium It is recommended to use Neuronal Medium (NM, Cat. #1521) for culturing HN-h in vitro.